Assays and other reactions involving droplets
US12331287B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 26, 2024 |
| Grant date | Jun 17, 2025 |
| Priority date | — |
| Expiry date | Nov 26, 2044 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/1006
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.