Patent · US Active

Systems and methods for allele enrichment using multiplexed blocker displacement amplification

US12331350B2 · kind B2 · utility

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3References
12Claims
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Key dates

Filing dateFeb 20, 2019
Grant dateJun 17, 2025
Priority date—
Expiry dateMay 19, 2042

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6858
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Provided herein are reagents and methods for simultaneously enriching many potential rare genetic variants at different genetic loci. The rare variants enriched can include single nucleotide polymorphisms (SNPs), single nucleotide variants, or small insertions and deletions. Embodiments of the invention include procedures for integration with downstream next generation sequencing (NGS) analysis. Embodiments of the invention include analysis of nonpathogenic SNPs for the determination of cell identity and detection of cell contamination using qPCR or NGS.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.