DNA sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators

US12331352B2 · kind B2 · utility

Assignee

• The Trustees of Columbia University in the City of New York · US

Inventors

• Jingyue Ju · Englewood Cliffs, US
• Dae Hyun Kim · Anmyeon-eup, KR
• Jia Guo · Chandler, US
• Qinglin Meng · Sunnyvale, US
• Zengmin Li · Great Neck, US
• Huanyan Cao · Jiangyin, CN

Key dates

• Filing date: Jan 14, 2022
• Grant date: Jun 17, 2025
• Expiry date: Jan 14, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2563/107
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

This invention provides a process for sequencing nucleic acids using 3′ modified deoxynucleotide analogues or 3′ modified deoxyinosine triphosphate analogues, and 3′ modified dideoxynucleotide analogues having a detectable marker attached thereto.