Chromatography ligand comprising domain c from Staphylococcus aureus protein a for antibody isolation

US12337293B2 · kind B2 · utility

Assignee

• Cytiva BioProcess R&D AB · SE

Inventors

• Martin Hall · Uppsala, SE
• Sture Larsson · Uppsala, SE
• Andreas Muranyi · Uppsala, SE
• Gustav Rodrigo · Uppsala, SE
• Jinyu Zou · Uppsala, SE
• Per-Mikael Aberg · Uppsala, SE

Key dates

• Filing date: Nov 4, 2022
• Grant date: Jun 24, 2025
• Expiry date: Feb 1, 2043

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/55
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.