Proteases having improved enzyme stability in washing and cleaning agents III

US12338469B2 · kind B2 · utility

Assignee

• Henkel AG & Co. KGaA · DE

Inventors

• Nina Mussmann · Willich, DE
• Susanne Wieland · Nievenheim, DE
• Christian Degering · Erkrath, DE
• Layla Fernandez · Cologne, DE
• Sabine Griemert · Monheim am Rhein, DE
• Barbara Peiffer · Rommerskirchen, DE
• Michael Puls · Monheim am Rhein, DE
• Inken Prueser · Dressendorf, DE

Key dates

• Filing date: Apr 14, 2020
• Grant date: Jun 24, 2025
• Expiry date: Feb 21, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y304/21062
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Proteases may include an amino acid sequence having at least 70% sequence identity with the amino acid sequence given in SEQ ID NO: 1 over its entire length and, in each case based on the numbering according to SEQ ID NO: 1, may include (i) amino acid substitutions, such as selected from amino acid substitutions 9T, 144K, 252T and 271E, at positions corresponding to positions 9, 144, 252 and 271, and (ii) at least one additional amino acid substitution at least at one of the positions corresponding to positions 53, 120, 131, 149, 159, 162, 166, 172, 189, 192, 211, 215, 217, 224 and 274. Production and use of said proteases are described herein. Proteases of this kind demonstrate very good stability with a good cleaning performance.