Method for determining the viability of cells

US12339215B2 · kind B2 · utility

Assignees

• COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES · FR
• IPRASENSE SAS · FR
• GlaxoSmithKline Intellectual Property Development Limited · GB

Inventors

• Cedric Allier · Grenoble, FR
• Lionel Herve · Meylan, FR
• Geoffrey Esteban · Nîmes, FR
• Martin Pisaneschi · Jacou, FR
• Melissa HILL · Granite Falls, US

Key dates

• Filing date: Apr 8, 2020
• Grant date: Jun 24, 2025
• Expiry date: Jun 20, 2041

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2510/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for determining a state of a cell, the cell being placed in a sample in contact with a culture medium, includes illuminating the sample with a light source and acquiring an image of the sample with an image sensor, the image sensor lying in a detection plane. From the acquired image, a position of the cell in a plane parallel to the detection plane is located and a refractive index or a relative refractive index of the cell is estimated, the relative refractive index corresponding to a refractive index of the cell relative to the refractive index of the culture medium. From the estimation, an index of interest of the cell is determined. From the index, a state of the cell among predetermined states is classified, the predetermined states including at least one apoptosis state and one living state.