Patent · US Active

Metabonomics-based tobacco leaf mildewing identification method

US12339260B2 · kind B2 · utility

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Key dates

Filing dateMar 29, 2023
Grant dateJun 24, 2025
Priority date
Expiry dateNov 28, 2043

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N2333/415
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

A metabonomics-based tobacco leaf mildewing identification method, which comprises: obtaining tobacco leaf samples of the same variety, and carrying out artificial mildewing on a certain amount of tobacco leaf samples to obtain mildewed tobacco leaves; measuring volatile and semi-volatile components in the tobacco leaves before and after the tobacco leaf samples are mildewed by adopting a solid phase microextraction-gas chromatography-mass spectrometry method; performing data processing on the collected mass spectrum data to obtain the proportion content of different types of compounds in the mildewed sample and the normal sample, and further obtaining the change difference of the content of volatile compounds in the tobacco leaf sample before and after mildewing; and establishing a tobacco leaf mildewing identification model according to the discrimination variables.

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