Patent · US Active

Membrane bound nucleic acid nanopores

US12344891B2 · kind B2 · utility

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25Claims
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Key dates

Filing dateAug 2, 2019
Grant dateJul 1, 2025
Priority date
Expiry dateNov 1, 2042

Classification

  • Technology area (CPC B)Performing Operations; Transporting
  • CPC primaryB82Y5/00
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A membrane-spanning nanopore is provided, the nanopore comprising: a. one or more polynucleotide strands that provide a scaffold component; and b. a plurality of polynucleotide strands that provide a plurality of staple components; wherein each of the plurality of staple components hybridise to the scaffold component; wherein the orientation of a major portion of at least one polynucleotide strand comprised within the scaffold component is substantially parallel to a planar surface of a membrane as well as embedded within and substantially coplanar with the membrane; wherein the nanopore defines a channel that is suitable for perforating the membrane; the channel having a longitudinal axis extending along the centre thereof and a minimum internal dimension perpendicular to the longitudinal axis of at least about 3 nm. A membrane comprising the nanopore, biological sensors comprising the membrane and a method for molecular sensing using the membrane are also disclosed.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.