Patent · US Active

Method for determining if a tumor has a mutation in a microsatellite

US12344900B2 · kind B2 · utility

0Cited by

2References

14Claims

0Family size

Assignee

The Board of Trustees of the Leland Stanford Junior University · US

Inventors

Giwon Shin · Stanford, US
Hanlee P. Ji · Stanford, US

Key dates

Filing date	Dec 17, 2020
Grant date	Jul 1, 2025
Priority date	—
Expiry date	Jan 12, 2043

Classification

Technology area (CPC G)Physics
CPC primaryG01N2800/52
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method for determining if a tumor has a mutation in a microsatellite is provided. In some embodiments, the method may comprise: (a) isolating genomic DNA from a tumor sample and a non-tumor sample from the same patient to produce: i. a sample of tumor DNA and ii. a sample of non-tumor DNA, respectively, (b) without pre-amplifying the tumor or non-tumor DNA, sequencing a plurality of microsatellite loci from both the tumor and non-tumor DNA using OS-seq to provide sequence reads, wherein the sequenced microsatellite loci comprise mononucleotide, dinucleotide, trinucleotide and tetranucleotide microsatellites loci, and (c) comparing the results.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.