Methods for assaying biological cells in a microfluidic device

US12345701B2 · kind B2 · utility

Assignee

• Bruker Cellular Analysis, Inc. · US

Inventors

• Yelena Bronevetsky · San Francisco, US
• Annamaria Mocciaro · San Francisco, US
• Guido K. Stadler · San Francisco, US
• Peter J. Beemiller · Emeryville, US
• Natalie C. Marks · Albany, US
• Duane Smith · Mayer, US
• Vincent Haw Tien Pai · Somerville, US
• Jason M. McEwen · Corona, US
• Amanda L. Goodsell · Emeryville, US
• John A. Tenney · Piedmont, US
• Thomas M. Vetterli · Berkeley, US
• Hansohl E. KIM · Emeryville, US

Key dates

• Filing date: Apr 27, 2021
• Grant date: Jul 1, 2025
• Expiry date: Feb 15, 2044

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2200/0647
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods are provided for the assay of secreted biomolecules using automated detection and characterization of micro-objects in a microfluidic device. The biomolecules can be secreted by cells, particularly immunological cells, such as T cells. The biomolecules being assayed can include cytokines, growth factors, and the like. Methods are also provided for assaying the cytotoxicity of a cell with respect to another, target cell. Also provided are kits and non-transitory computer-readable media in which programs are stored for causing a system comprising a computer to perform automated methods for detecting secreted biomolecules and/or cytotoxicity in a microfluidic device.