Autonomous denudation in an intelligent automated in vitro fertilization and intracytoplasmic sperm injection platform
US12349940B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 2, 2024 |
| Grant date | Jul 8, 2025 |
| Priority date | — |
| Expiry date | Feb 2, 2044 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06V2201/03
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for automated, artificial-intelligence-based denudation includes scanning, robotically, a dish containing a cumulus-oocyte-complex (COC) using an imaging system and an artificial intelligence/machine learning system (AI/ML system) to create an image object. The imaging system includes a microscopy system, a camera system, and a lighting system. The dish includes an enzyme to remove cumulus cells from an oocyte among the COC. The method includes identifying the COC within the image object, and a location, based on comparing the image object to a threshold using the AI/ML system. The threshold is an optical pattern with a probability of corresponding to a COC mass. The method includes instructing a robotic pipettor to collect the COC at the location. The method includes iteratively collecting the COC within the robotic pipettor and expelling the COC to return it to the dish until the AI/ML system confirms sufficient removal of cumulus and corona cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.