Patent · US Active

Gene sequencing chip and gene sequencing method

US12350636B2 · kind B2 · utility

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12Claims
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Key dates

Filing dateFeb 28, 2022
Grant dateJul 8, 2025
Priority date
Expiry dateFeb 20, 2044

Classification

  • Technology area (CPC B)Performing Operations; Transporting
  • CPC primaryB01L2400/0427
  • WIPO fieldChemical engineering
  • WIPO sectorChemistry

Abstract

A gene sequencing chip is provided, which includes: an upper substrate including a plurality of liquid inlets for inletting liquid drops; a lower substrate opposite to the upper substrate and spaced therefrom by a gap, the gap being provided for allowing the liquid drops to move therein, the lower substrate including a liquid drop operation region, the liquid drop operation region including a manipulation electrode array. The manipulation electrode array includes multiple first manipulation electrode array for preparing a gene library, multiple second manipulation electrode array for sequencing the gene library which is prepared, each first sub-array being adjacent to one of the multiple second manipulation electrode array. Based on the gene sequencing chip provided in this disclosure, operations to tiny liquid drops such as movement, fusion and splitting can be accurately manipulated by using digital microfluidic techniques, and all steps of the gene sequencing from library preparation to gene sequencing can be completed on one chip.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.