Patent · US Active

Recombinant Escherichia coli for producing l-tyrosine and application thereof

US12351795B2 · kind B2 · utility

0Cited by

1References

10Claims

0Family size

Assignee

Jiangnan University · CN

Inventors

Jingwen Zhou · Wuxi, CN
Jian Chen · Wuxi, CN
Jurong Ping · Wuxi, CN
Weizhu Zeng · Wuxi, CN

Key dates

Filing date	Jan 31, 2024
Grant date	Jul 8, 2025
Priority date	—
Expiry date	Jan 31, 2044

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY02A50/30
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Disclosed is recombinant Escherichia coli for producing L-tyrosine and application thereof, and belongs to the technical fields of genetic engineering and bioengineering. According to the present disclosure, genes aroP and tyrP are knocked out, expresses the endogenous gene yddG of E. coli, then heterologously expresses fpk from Bifidobacterium adolescentis, expresses the endogenous genes ppsA and tktA of E. coli, and then expresses aroGfbr and tyrAfbr. Knocking out tyrR, trpE and pheA, so that the synthesis flux of L-tyrosine is increased. Finally, an endogenous gene poxB is knocked out to realize stable fermentation performance at high glucose concentration.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.