Recombinant adeno-associated virus particle purification with multiple-step anion exchange chromatography
US12351832B2 · kind B2 · utility
Assignees
Inventor
Key dates
| Filing date | Nov 15, 2021 |
| Grant date | Jul 8, 2025 |
| Priority date | — |
| Expiry date | Jul 7, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2750/14151
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A method for obtaining purified recombinant Adeno-Associated Virus particles (rAAV) is provided. The method includes steps of: a) performing a depth filtration of a cell lysate or a culture supernatant obtained from cells producing rAAV particles, whereby a rAAV-containing clarified composition is provided; b) submitting the rAAV-containing clarified composition to a first step of anion-exchange chromatography on a chromatographic support wherein the rAAV-containing fraction is collected, whereby a first rAAV enriched composition is provided; c) submitting the first rAAV enriched composition at least once to a second step of anion-exchange chromatography on a chromatographic support wherein the rAAV-containing fraction is collected, whereby a second rAAV enriched composition is provided; d) submitting the second rAAV enriched composition to a step of tangential flow filtration to provide purified recombinant Adeno-Associated Virus particles (rAAV).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.