Group of UDP-glycosyltransferase for catalyzing carbohydrate chain elongation and application thereof

US12356911B2 · kind B2 · utility

Assignee

• GSYNBIOT (SHANGHAI) CO., LTD · CN

Inventors

• Zhihua Zhou · Shanghai, CN
• Wei Wei · Lo Wu, CN
• Xing Yan · Aberdeen, GB
• Chengshuai Yang · Shanghai, CN
• Chaojing Li · Shanghai, CN
• Yongjun Wei · Hangzhou City, CN
• Pingping Wang · Shanghai, CN

Key dates

• Filing date: Oct 28, 2022
• Grant date: Jul 15, 2025
• Expiry date: Oct 28, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P33/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a group of glycosyltransferase, and an application thereof. Specifically, provided is using glycosyltransferase GT29-32, GT29-33, GT29-34, GT29-4, GT29-5, GT29-7, GT29-9, GT29-11, GT29-13, GT29-17, GT29-18, GT29-19, GT29-20, GT29-21, GT29-22, GT29-23, GT29-24, GT29-25, GT29-36, GT29-37, GT29-42, GT29-43, GT29-45, GT29-46, PNUGT29-1, PNUGT29-2, PNUGT29-3, PNUGT29-4, PNUGT29-5, PNUGT29-6, PNUGT29-7, PNUGT29-8, PNUGT29-9, PNUGT29-14, and PNUGT29-15, as well as derived polypeptides thereof to catalyze the first glycosyl at position C-20, the first glycosyl at position C-6, and the first glycosyl at position C-3 of a tetracyclic triterpene compound substrate to elongate a carbohydrate chain, thereby obtaining a catalytic reaction of ginsenoside products such as ginsenoside Rg3, ginsenoside Rd, ginseno-side Rb 1, ginsenoside Rb3, saponin DMGG, saponin DMGX, gypenoside LXXV, gypenoside XVII, gypenoside XIII, gypenoside IX, notoginsenoside U, and notoginsenoside R1, notoginsenoside R2, notoginsenoside R3, 3-0-13-(D-xylopyranosyl)-13-(D-glucopyra-nosyl)-PPD, 3-0-13-(D-xylopyranosyl)-13-(D-glucopyranosyl)-CK, 20-O-Glucosylginsenoside Rf, and Ginsenoside F3. G…