Compositions and methods for modifying a target nucleic acid

US12359179B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Alexander Marson · San Francisco, US
• Theodore Lee Roth · San Francisco, US
• Daniel Bryan Goodman · Cambridge, US
• David-Huy Nhu Nguyen · Oakland, US
• Francis C. Szoka, Jr. · San Francisco, US

Key dates

• Filing date: Dec 12, 2019
• Grant date: Jul 15, 2025
• Expiry date: Aug 14, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2800/80
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The disclosure provides compositions and methods for modifying a target nucleic acid. In some embodiments, a composition can include a targetable nuclease, a DNA-binding protein, and a donor template comprising a homology directed repair (HDR) template and one or more DNA-binding protein target sequences. In some embodiments, a composition can include a Cas protein, one or more single guide RNAs (sgRNAs), and an anionic polymer.