T4 DNA ligase variants with increased resistance to salt
US12359185B2 · kind B2 · utility
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Key dates
| Filing date | May 10, 2023 |
| Grant date | Jul 15, 2025 |
| Priority date | — |
| Expiry date | Jun 4, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y605/01001
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A number of T4 DNA ligase mutants exhibiting enhanced ligation activity in the presence of high salt concentrations compared to the wild-type ligase were engineered, characterized, and selected via gel electrophoresis of ligation products from a standard ligation assay. Ligase catalyzes the formation of phosphodiester bonds between the 5′ and 3′ ends of complementary cohesive ends or blunt ends of duplex DNA, a process that is vital to numerous molecular biology processes including cloning and sequencing.
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