Guided magnetic nanostructures for targeted and high-throughput intracellular delivery

US12359220B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Paul S. Weiss · Los Angeles, US
• Hsian-Rong Tseng · Los Angeles, US
• Xiaobin Xu · White Plains, US
• Natcha Wattanatorn · Los Angeles, US
• Qing Yang · Pleasanton, US
• Steven J. Jonas · Bloomfield Hills, US

Key dates

• Filing date: Jan 29, 2019
• Grant date: Jul 15, 2025
• Expiry date: May 14, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2310/141
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A method of transporting biomolecular cargo intracellularly into cells includes the operations of providing magnetic nanostructures (e.g., nanospears, nanostars, nanorods, and other nanometer-sized structures) carrying the biomolecular cargo thereon and applying an external magnetic field to move the magnetic nanostructures into physical contact with at least some of the cells (or the cells into the magnetic nanostructures). The magnetic nanostructures move into physical contact with a single cell, a subset of cells, or all cells. The external magnetic field may be applied by a moving permanent magnet although an electromagnetic may also be used. The biomolecular cargo may include a molecule, a plurality of molecules, or higher order biological constructs. For example, the biological construct may include DNA plasmids, small interfering RNA, proteins, or targeted nuclease gene-editing cargo such as zinc-finger nucleases, transcription activator-like effector nucleases, Cas9 protein, Cas9 mRNA, and associated guide RNA sequences.