Membrane protease-based methods for detection of bacteria
US12359240B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Apr 27, 2020 |
| Grant date | Jul 15, 2025 |
| Priority date | — |
| Expiry date | May 15, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/255
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides a method for detecting bacteria. The method utilises a peptide that forms a complex with a conjugated reporter polymer and is susceptible to cleavage by one or more proteases on the surface of a bacteria. Presence or absence of the bacteria can be determined by assessing the optical absorption and/or colour and/or photoluminescence (e.g. fluorescence) of the conjugated reporter polymer, which may undergo a conformational change after binding with the to the cleaved peptide substrate. Specifically, the peptide substrate may comprise a cleavage site for digestion by the protease, and the protease may be an omptin protease. The conjugated reporter polymer may be selected from a polythiophene, a poly(1,4-phenylene vinylene) (PPV), a poly(1,4-phenylene) (PPP), a polyfluorenes (PFO), a nitrogen-containing polymer such as polyquinoline, poly(2,5-pyridinevinylene) (PPyV), 1,3,4-oxadiazole, and poly(9-vinylcarbazole) (PVK), and a polypyrrole. The method may be used to detect contamination in food or water, or as a clinical and/or diagnostic test.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.