Primer, device for producing double-stranded DNA using primer, and method for producing double-stranded DNA using primer
US12359254B2 · kind B2 · utility
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13Claims
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Key dates
| Filing date | Jul 31, 2020 |
| Grant date | Jul 15, 2025 |
| Priority date | — |
| Expiry date | Aug 13, 2042 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/55
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
where A1 represents —S—, —S—S—, or —Se—, B represents a base, and R1 represents a decomposable protecting group, and the symbol * represents a bond to a sugar of an adjacent nucleotide. A device for producing double-stranded DNA includes: a forward primer and a reverse primer, having a structure represented by formula (1); a PCR device for forming double-stranded DNA with 3′-recessed ends by performing multiple cycles of PCR by using a template DNA as a template; Klenow fragment for making the 3′ ends blunt; and a photoirradiation unit for deprotecting R1 and forming a sticky end with a 3′-protruding end.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.