Tiled ClickSeq for targeted virus whole genome sequencing
US12359266B2 · kind B2 · utility
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Key dates
| Filing date | Jun 18, 2021 |
| Grant date | Jul 15, 2025 |
| Priority date | — |
| Expiry date | Sep 16, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/156
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention includes a method and kit for single-primer tiled sequencing comprising: reverse transcribing a target nucleic acid with multiple tiled primers spaced along the genome, each only targeting one annealing site to form amplicons in the presence of terminating 3′ azido nucleotides to incorporate the terminating 3′ azido nucleotides into the cDNA; click-ligating a downstream primer onto tire 3′ azido terminated cDNA such that a second template-specific primer is not required; amplifying the click-ligated cDNA; and sequencing the amplicons.
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