Rapid detection method for abrin toxin
US12360116B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 12, 2024 |
| Grant date | Jul 15, 2025 |
| Priority date | — |
| Expiry date | Sep 12, 2044 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A rapid detection method for abrin toxin is provided. The rapid detection method for abrin toxin includes the following steps: reacting an oligonucleotide chain substrate labeled with a fluorescent group and a quenching group, a buffer solution, a BSA solution, a to-be-detected sample and sterile water, incubating at a constant temperature, detecting a fluorescence signal value, and determining whether the abrin toxin exists based on a difference between a change in final fluorescence signal value and a change in final fluorescence signal value of a negative control; where the sample is judged as a positive sample when an average value of the detected final fluorescence signal values is greater than an average value of the final fluorescence signal values of the negative control by +3 times standard deviation. According to the detection method, the direct addition of the sample into the reaction system can reach the sensitivity of 30 ng/mL.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.