System for identifying and targeting individual cells within a heterogeneous population for selective extraction of cellular content
US12364982B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 29, 2019 |
| Grant date | Jul 22, 2025 |
| Priority date | — |
| Expiry date | Sep 19, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/1028
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A system for identifying and targeting individual cells within a cell population for selective extraction of cellular content. The system includes a digital microfluidic device having at least one hydrophilic site for receiving cells, an imaging system including a stage for receiving the digital microfluidic device and an imaging module for identifying at least one targeted cell among the cells at the at least one hydrophilic site and a pulsed laser source for lysing the targeted cell to produce a lysate. A control system controls the pulsed laser source, the imaging system and the digital microfluidic device and is programmed for coordinating steps of i) moving droplets on the digital microfluidic device, ii) selecting the targeted cell to be lysed located at the hydrophilic site, iii) illuminating the selected targeted cell by the pulsed laser source to lyse the selected targeted cell to produce lysate, and iv) collecting the lysate.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.