Method of purifying a protein from fermentation solids under desorbing conditions

US12365866B2 · kind B2 · utility

Assignee

• BASF SE

Inventors

• Katrin Treier-Marxen · Ludwigshafen, DE
• Petra Deckert · Bammental, DE
• Andreas Schaedler · Stelle, DE
• Vaidotas Navickas · Mannheim, DE
• Volker Wengert · Worms, DE
• Thomas Kaeding · Mannheim, DE

Key dates

• Filing date: Dec 8, 2016
• Grant date: Jul 22, 2025
• Expiry date: Dec 8, 2036

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01D2315/16
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

The present invention is directed to a method of purifying a protein of interest from the particulate matter of a fermentation broth comprising the step of purifying the protein of interest from the particulate matter of the fermentation broth, wherein the step of purifying the protein of interest from the particulate matter of the fermentation broth comprises one or more washing steps, which comprises contacting the particulate matter with a washing solution comprising one or more conditions that favor the solubilization of the protein of interest and/or the desorption of the protein of interest from the particulate matter.