Double-stranded splint adaptors with universal long splint strands and methods of use
US12365892B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 12, 2023 |
| Grant date | Jul 22, 2025 |
| Priority date | — |
| Expiry date | Sep 12, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2565/507
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure provides compositions comprising nucleic acid double-stranded splint adaptors, including kits, and methods that employ the double-stranded splint adaptors. The double-stranded splint adaptors (200) can be used in a one-pot, multi-enzyme reaction to introduce one or more new adaptor sequences into a library molecule. The double-stranded splint adaptor (200) comprises a first splint strand (long splint strand (300)) and a second splint strand (short splint strand (400)), where the first and second splint strands are hybridized together to form the double-stranded splint adaptor (200) having a double-stranded region and two flanking single-stranded regions. The second splint strand (400) carries the new adaptor sequence(s) to be introduced, such as for example a universal binding sequence, an index sequence and/or a random sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.