Immortalization of splenic and peripheral blood macrophages using a multi-cistronic v-raf/v-myc lentivirus
US12365917B2 · kind B2 · utility
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Key dates
| Filing date | Mar 19, 2019 |
| Grant date | Jul 22, 2025 |
| Priority date | — |
| Expiry date | Sep 14, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/70596
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Vectors and methods are disclosed for immortalizing mammalian cells by co-expression of v-raf and v-myc proteins. A replication-defective viral vector is used for improved safety. The vector comprises an optional marker gene, and is especially useful for producing an immortalized macrophage by a method that involves contacting the vector with a monocyte, proliferatively growing the monocyte, growing the monocytic cell on a solid surface, and then growing the monocytic cell on a porous surface. An immortalized macrophage is also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.