Methods and compositions to promote targeted genome modifications using HUH endonucleases
US12371695B2 · kind B2 · utility
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Key dates
| Filing date | Jul 31, 2020 |
| Grant date | Jul 29, 2025 |
| Priority date | — |
| Expiry date | Apr 6, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This disclosure provides compositions and methods for improving site-directed integration of nucleic acids using RNA-guided nucleases coupled with HUH endonucleases. In one aspect, this disclosure provides a ribonucleoprotein comprising: (a) a re-combinant polypeptid comprising: (i) an amino acid sequence encoding a Cas12a nuclease; (ii) an amino acid sequence encoding a linker; and (iii) an amino acid sequence encoding a HUH nuclease; and (b) at least one guide nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.