Method for detecting a binding of antibodies from a patient sample to double-stranded DNA using Crithidia luciliae cells and fluorescence microscopy

US12372532B2 · kind B2 · utility

Assignee

• EUROIMMUN Medizinische Labordiagnostika AG · DE

Inventors

• Stefan Gerlach · Groß, DE
• Jens Krauth · Stade, DE
• Christian Marzahl · Erlangen, DE
• Christopher Krause · Bad Honnef, DE
• Maick Danckwardt · Rondeshagen, DE
• Melanie Hahn · Stockelsdorf, DE
• Jörn Voigt · Lübeck, DE

Key dates

• Filing date: Sep 17, 2021
• Grant date: Jul 29, 2025
• Expiry date: Oct 9, 2043

Classification

• Technology area (CPC G): Physics
• CPC primary: G06T2207/30024
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

There is proposed a method for detecting a binding of autoantibodies from a patient sample to double-stranded deoxyribonucleic acid using Crithidia luciliae cells by means of fluorescence microscopy, including the steps of: provision of a substrate which has multiple Crithidia luciliae cells, incubation of the substrate with the patient sample which potentially has the autoantibodies, incubation of the substrate with secondary antibodies which have each been labelled with a preferably green fluorescent dye, acquisition of a fluorescence image of the substrate, identification by means of a first pretrained convolutional neural network of respective sub-images in the one fluorescence image that each represent a Crithidia luciliae cell, furthermore respective processing of at least one subset of the respective sub-images by means of a second pretrained convolutional neural network for determining respective binding measures which indicate a respective extent of a binding of autoantibodies in a respective kinetoplast region of a respective Crithidia luciliae cell of a respective sub-image, and determination of an overall binding measure with regard to a binding of autoantibodies from t…