Automatic focusing method for fluorescence imaging device
US12372740B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 8, 2023 |
| Grant date | Jul 29, 2025 |
| Priority date | — |
| Expiry date | Mar 21, 2044 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02B20/00
- WIPO fieldOptics
- WIPO sectorInstruments
Abstract
The present application provides an automatic focusing method for a fluorescence imaging device, including: S1, obtain a relationship function curve Z-Fv; S2, Gaussian fitting is performed on the relationship function curve Z-Fv, to obtain a standard Gaussian function f(x); S3, the object lens is arranged at three different travel coordinates, to obtain three groups of coordinate-clarity data (Z, Fv); S4, any two groups of the data are taken from the three groups of the coordinate-clarity data (Z, Fv) obtained in S3 and perform the Gaussian fitting with a standard deviation σ, to obtain a Gaussian function f1(x); and S5, an average value μ1 of the Gaussian function f1(x) is calculated, as a preferred travel coordinate Z′ for this time of the focusing object lens. The present application solves problems of large focusing clarity error and slow focusing speed of the fluorescence imaging device in existing technologies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.