In-vitro culture, induction, activation and cryopreservation method and cell bank establishment for immune cells
US12376584B2 · kind B2 · utility
Inventors
Key dates
| Filing date | May 26, 2020 |
| Grant date | Aug 5, 2025 |
| Priority date | — |
| Expiry date | Jun 5, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/52
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses an in-vitro culture, induction, activation and cryopreservation method and cell bank establishment for immune cells. The method includes the follows: using a dedicated amplification medium of immune cells to perform first-stage amplification culture on mononuclear cells to obtain preliminarily amplified immune cells; using a dedicated induction medium of immune cells to perform second-stage induction and amplification culture on the preliminarily amplified immune cells to obtain induced immune cells; using a dedicated activation medium of immune cells to perform third-stage activation and amplification culture on the induced immune cells to obtain a large number of immune cells with activation functions; using a dedicated cryopreserving fluid of immune cells to cryopreserve the immune cells to obtain cryopreserved immune cells; and performing preservation according to ABO/RH typing and HLA typing; and establishing an information file of immune cells for retrieval to construct an immune cell bank.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.