Recombinant collagen and preparation method and use thereof

US12378303B2 · kind B2 · utility

Assignee

• JIANGSU TRAUTEC MEDICAL TECHNOLOGY CO., LTD. · CN

Inventors

• Jiajia Li · Changzhou City, CN
• Liping Wang · Huaibei, CN
• Huimin Liu · Kunshan, CN
• Wenwen Jiang · 东风镇, CN
• Chenming Qian · Changzhou City, CN
• Pengfei Cheng · Shanghai, CN
• Song Qian · Changzhou City, CN

Key dates

• Filing date: Nov 23, 2022
• Grant date: Aug 5, 2025
• Expiry date: Nov 23, 2042

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Medical technology
• WIPO sector: Instruments

Abstract

A recombinant collagen and a preparation method and use thereof, and in particular, a full-length collagen α1 chain produced through recombinant expression, and a preparation method and use thereof are provided. When a variant (which is denoted as α1 (I) M1) of a human type I collagen α1 chain (α1 (I) chain) and a variant (which is denoted as α1 (II) M6) of a human type II collagen α1 chain (α1 (II) chain) constructed in the present disclosure are produced through recombinant expression in Pichia pastoris, a main degradation product (a main degradation band) in substantially the same proportion as a target product (a target band) of a full-length α1 chain occurring during the recombinant expression of native full-length α1 (I) chain and α1 (II) chain is eliminated, and a yield of the target product is improved.