Lipid nanodiscs solubilized through poly(acrylic acid-co-styrene) copolymers

US12378337B2 · kind B2 · utility

Assignees

• The Board of Trustees of the Leland Stanford Junior University · US
• The Regents of the University of California · US
• AARHUS UNIVERSITET · DK

Inventors

• Eric A. Appel · Palo Alto, US
• Anton Smith · San Francisco, US
• Henriette E. Autzen · San Francisco, US
• Yifan Cheng · San Francisco, US

Key dates

• Filing date: Jun 19, 2020
• Grant date: Aug 5, 2025
• Expiry date: Jun 29, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C08F2438/03
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Provided herein are compositions including lipids and copolymers in the form of a nanodisc assembly. The subject copolymers include monomer units of styrene and monomer units selected from acrylic acid and an acrylic acid derivative. In certain cases, the copolymer is a copolymer of styrene and acrylic acid. Also provided herein, is an aqueous solution comprising the subject composition. Also provided herein, are methods for producing a nanodisc assembly, including incubation of a lipid and a subject copolymer. Further provided herein, are methods for solubilizing a membrane protein in an aqueous solution, wherein the method includes forming a nanodisc assembly of a lipid bilayer having one or more membrane proteins embedded therein, and a subject copolymer. Also provided are methods of solubilizing a hydrophobic constituent in an aqueous solution, including forming a nanodisc assembly of a lipid, a hydrophobic constituent, and a subject copolymer.