Plant genome modification using guide RNA/Cas endonuclease systems and methods of use
US12378566B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Nov 15, 2022 |
| Grant date | Aug 5, 2025 |
| Priority date | — |
| Expiry date | Nov 15, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/3341
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.