Nucleic acid clonal amplification and sequencing methods, systems, and kits
US12378593B2 · kind B2 · utility
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20Claims
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Key dates
| Filing date | Jan 15, 2021 |
| Grant date | Aug 5, 2025 |
| Priority date | — |
| Expiry date | May 8, 2044 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6858
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure provides methods and systems for processing nucleic acid samples. Methods for processing a nucleic acid sample may comprise providing a double-stranded nucleic acid molecule comprising a partially denaturable region; partially denaturing the partially denaturable region of the double-stranded nucleic acid molecule, thereby generating a region comprising two single strands; and hybridizing a priming sequence to a sequence of one of the single strands. The methods described herein may facilitate amplification without the need for a multitude of complex steps or numerous reagents.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.