Enhancing agent and kit for enhancing nucleic acid amplification reaction and method for performing nucleic acid amplification reaction
US12385084B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 17, 2020 |
| Grant date | Aug 12, 2025 |
| Priority date | — |
| Expiry date | Feb 17, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2561/113
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure discloses an enhancing agent and a kit for enhancing nucleic acid amplification reaction and a method for performing nucleic acid amplification reaction, relating to the field of biotechnology. The enhancing agent disclosed in the present disclosure has a domain capable of binding to fluorescent dye and a quenching group for quenching fluorescence emitted by the fluorescent dye bound to the domain binds. As the binding affinity between the above domain and the fluorescent dye is lower than the binding affinity between a target amplification product of nucleic acid amplification reaction and the fluorescent dye, the enhancing agent not only can reduce inhibition of high-concentration fluorescent dye to amplification reaction, but also can enhance signal intensity of the fluorescent dye in the nucleic acid amplification reaction based on microfluidic chip, improving reliability of application of the fluorescent dye as amplification indicator on the microfluidic chip.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.