Immobilization in flow cells

US12385091B2 · kind B2 · utility

Assignee

• Illumina, Inc. · US

Inventors

• Jeffrey S. Fisher · San Francisco, US
• Tarun Khurana · San Diego, US
• Mathieu Lessard-Viger · San Diego, US
• Clifford Lee Wang · Redwood City, US
• Yir-Shyuan Wu · Fremont, US

Key dates

• Filing date: Dec 11, 2020
• Grant date: Aug 12, 2025
• Expiry date: Jan 2, 2043

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2001/4038
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

In an example, a target material is immobilized on two opposed sequencing surfaces of a flow cell using first and second fluids. The first fluid has a density less than a target material density and the second fluid has a density greater than the target material density; or the second fluid has a density less than the target material density and the first fluid has a density greater than the target material density. The first fluid (including the target material) is introduced into the flow cell, whereby at least some of the target material becomes immobilized by capture sites on one of the sequencing surfaces. The first fluid and non-immobilized target material are removed. The second fluid (including target material) is introduced into the flow cell, whereby at least some of the target material becomes immobilized by capture sites on another of the sequencing surfaces.