Cleavable fluorescent tyramide for sensitive and multiplexed analysis of biological samples
US12385923B2 · kind B2 · utility
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10Claims
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Key dates
| Filing date | Nov 11, 2019 |
| Grant date | Aug 12, 2025 |
| Priority date | — |
| Expiry date | Nov 30, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6439
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided herein are methods for multiplexed in situ analysis of biomolecules in a tissue. In particular, provided herein are methods for multiplexed single-cell in situ protein and nucleic acid profiling in fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.