Genetically modified nitrogen fixing bacteria and uses thereof
US12391627B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Sep 18, 2020 |
| Grant date | Aug 19, 2025 |
| Priority date | — |
| Expiry date | Jan 31, 2044 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/065
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A genetically modified bacterium for excreting fixed nitrogen (in the form of ammonia) is disclosed. The bacterium can be made by deleting at least a portion of the nifL gene of a diazotrophic γ-proteobacterium, and inserting a promoter sequence into the diazotrophic γ-proteobacterium genome that is placed and oriented to direct transcription of the rnf1 gene complex. The resulting genetically modified bacterium excretes ammonia constitutively and at a greater rate than the wild type bacterium, and can be used to make biofertilizers to stimulate plant growth. The biofertilizers may contain a culture of the bacteria, or a co-culture of the bacteria and a mycorrhizal fungus.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.