Composition and kit for detecting mycoplasma

US12391998B2 · kind B2 · utility

Assignees

• Jiangsu Academy of Agricultural Sciences · CN
• GUOTAI (TAIZHOU) CENTER OF TECHNOLOGY INNOVATION FOR VETERINARY BIOLOGICALS, TAIZHOU · CN

Inventors

• Zhixin Feng · Nanjing, CN
• Yanfei Yu · Meekrap-oord, CN
• Jia-Yu Wang · Amherst, US
• Yanna Wei · Nanjing, CN
• Guoqing Shao · Nanjing, CN
• Yuzi Wu · Nanjing, CN
• Maojun Liu · Nanjing, CN
• Qiyan Xiong · Nanjing, CN
• Li-Ting Wang · Meekrap-oord, CN
• Beibei Liu · Nanhu, CN

Key dates

• Filing date: Oct 10, 2024
• Grant date: Aug 19, 2025
• Expiry date: Oct 10, 2044

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/16
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A composition and a kit for detecting mycoplasma are provided. The composition for detecting mycoplasma is an aqueous solution including a primer M-F, a primer M-R, and a probe M-P. A sequence of the M-F is shown in SEQ ID NO: 1. A sequence of the M-R is shown in SEQ ID NO: 2. A nucleotide sequence of the probe M-P is shown in SEQ ID NO: 3, and includes a fluorophore FAM linked at a 5′ terminus and a quencher BHQ1 linked at a 3′ terminus. The composition exhibits high sensitivity, strong specificity, and a wide detection range when used in the detection of mycoplasma.