Method for re-using test probe and reagents in biochemical assay based on interferometry
US12399173B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 7, 2020 |
| Grant date | Aug 26, 2025 |
| Priority date | — |
| Expiry date | Feb 1, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54373
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention is directed to biochemical assay methods, which re-use a hapten-immobilized test probe and reagents for quantitating an analyte or measuring kinetic binding in different samples, anywhere from about 3 to 20 times, while maintaining acceptable assay performance. The methods use a conjugate solution either comprising (i) an anti-hapten antibody and a capture antibody against an analyte, or (ii) an anti-hapten antibody and streptavidin, in each cycle and regenerate the hapten-coated test probe by dipping the test probe in an acidic solution having pH about 1-4, optionally further dipping in a DMSO solution, after the completion of each cycle of reaction. The robustness of the hapten-immobilized solid phase allows utilization of denaturation reagents for efficient elution of the immune complexes after each cycle without compromising the binding activity of the hapten on the solid phase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.