Specimen enrichment for optical tomography cell analysis
US12399178B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 29, 2022 |
| Grant date | Aug 26, 2025 |
| Priority date | — |
| Expiry date | Feb 19, 2043 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N15/1433
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for enhancing gating performance of a cell sorter to prepare an enriched specimen for optical tomography cell analysis includes introducing a specimen into a FACS to generate 2D event data; generating a first scatterplot of the 2D data; identifying target objects; constructing a boundary within the first scatterplot to produce a first gate; counting target objects within the first gate; comparing the number of target objects within the first gate to a first predetermined value and adjusting the first gate as necessary. A boundary around a set of target objects is constructed in a second scatterplot to produce a subset second gate and target objects within the second gate are counted and the count compared to a second predetermined value. When a boundary around target objects meets specifications the first and second gates are stored in memory and used to enrich patient specimens.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.