In situ code design methods for minimizing optical crowding
US12400733B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 6, 2024 |
| Grant date | Aug 26, 2025 |
| Priority date | — |
| Expiry date | Sep 6, 2044 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/08
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
Methods and systems for performing in situ decoding are described that minimize optical crowding, thereby improving decoding accuracy. The methods may comprise, e.g., receiving images of a biological sample acquired during a cyclical decoding process; detecting a series of detectable signals (ON signals) or absence thereof (OFF signals) at one or more locations in the biological sample corresponding to one or more barcoded target analytes; determining a code word based on the series of ON and OFF signals that corresponds to a barcode for each of the one or more barcoded target analytes, where the one or more code words are assigned to the one or more barcoded target analytes based on a minimax decision rule to minimize a density of ON signals detected in the images of the series of images; and identifying the one or more barcoded target analytes based on the one or more determined code words.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.