Method of cell lysis

US12404501B2 · kind B2 · utility

Assignee

• Evonik Operations GmbH · DE

Inventors

• Steliyana Dimitrova · Babenhausen, DE
• Patrik Stenner · Hanau, DE
• Silvia BLANK-SHIM · Aschaffenburg, DE
• Tobias Müller · Hirschberg, DE

Key dates

• Filing date: Jun 2, 2022
• Grant date: Sep 2, 2025
• Expiry date: Jul 16, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12R2001/865
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a method of cell lysis for forming at least one pore in the cell wall and/or cell membrane of a cell in an aqueous medium for release of at least one intracellular target protein and/or lipid from the cell into the aqueous medium, including subjecting the cell with the intracellular target protein and/or lipid in the aqueous medium to an electrohydraulic comminution; and separating the target protein and/or lipid from the cell.The electrohydraulic comminution is brought about by a pulse current source. The cell in the aqueous medium is subjected to electrical impulse discharges between at least two electrodes of an underwater radio path of a container at a specific operating voltage, impulse energy, and pulse frequency, and at least one pore is formed in the cell wall and/or cell membrane.