Enhancing agents for improved cell transfection and/or rAAV vector production
US12404526B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 6, 2018 |
| Grant date | Sep 2, 2025 |
| Priority date | — |
| Expiry date | Jun 6, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2750/14151
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided are compositions and methods of transducing/transfecting cells with a molecule, such as a nucleic acid (e.g., plasmid), at high efficiency. High efficiency transduced/transfected cells can, when transduced with a nucleic acid that encodes a protein or comprises a sequence that is transcribed into a transcript of interest, produce high amounts of protein and/or transcript. High efficiency transduced/transfected cells can, when transduced with plasmids comprising (i) nucleic acids encoding AAV packaging proteins and/or nucleic acids encoding helper proteins; and (ii) a transgene that encodes a protein or is transcribed into a transcript of interest; produce high amounts of recombinant rAAV vector.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.