Detection of methylation status of a DNA sample
US12404546B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 13, 2024 |
| Grant date | Sep 2, 2025 |
| Priority date | — |
| Expiry date | Sep 13, 2044 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6851
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods for quantitative analysis of methylation content of a test sample. The method employs an internal control within the same test sample and an external control within a reference sample to reduce sources of random variability. The internal control corrects for variability arising from conducting separate amplifications as occurs in an external control, and the external control corrects for variability between amplification at different loci as occurs with the internal control. The steps for processing and analyzing the test sample can occur in a single reaction vessel facilitating automation of the assay. Information regarding methylation content generated by the methods can be used in diagnosing and treatment of disorders and for forensic purposes among other applications.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.