Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes

US12404549B2 · kind B2 · utility

Assignee

• PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

• Morassa Mohseni Middleton · San Diego, US
• Mark C. Wallen · San Diego, US
• Pinar Iyidogan · San Diego, US
• Michael James Schmidt · San Diego, US
• Brittany A. Rohrman · San Diego, US
• Ying Lin Liu · San Diego, US
• Fabian Block · San Diego, US
• Arnold Oliphant · Sunnyvale, US

Key dates

• Filing date: Jan 4, 2022
• Grant date: Sep 2, 2025
• Expiry date: Jun 19, 2044

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6832
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods, compositions, kits and apparatuses that include a fluid, the fluid containing a ternary complex and Li+, wherein the ternary complex includes a primed template nucleic acid, a polymerase, and a nucleotide cognate for the next correct base for the primed template nucleic acid molecule. As an alternative or addition to Li+, the fluid can contain betaine or a metal ion that inhibits polymerase catalysis such as Ca2+. In addition to Li+, the fluid can contain polyethylenimine (PEI) with or without betaine.