Transaminase mutant and use thereof

US12410413B2 · kind B2 · utility

Assignee

• ASYMCHEM LABORATORIES (TIANJIN) CO., LTD. · CN

Inventors

• Hao Hong · Tianjin, CN
• Gage James · 梅厂镇, CN
• Yi Xiao · Chesterfield, US
• Yulei Ma · Tianjin, CN
• Na Zhang · Nanhu, CN
• Xuecheng Jiao · Tianjin, CN
• Huiyan Mu · Tianjin, CN
• Yibing Cheng · Tianjin, CN
• Shan CAO · Tianjin, CN

Key dates

• Filing date: Oct 28, 2019
• Grant date: Sep 9, 2025
• Expiry date: May 3, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y206/01
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided are a transaminase mutant and use thereof. The transaminase mutant has an amino acid sequence obtained by mutation of an amino acid sequence shown in SEQ ID NO:1, the mutation at least includes one of the following mutation site combinations: T7C+S47C, Q78C+A330C, V137C+G313C, A217C+Y252C and L295C+C328C; or the transaminase mutant has an amino acid sequence which has the mutation sites in the mutated amino acid sequence and has 80% or more identity with the mutated amino acid sequence. The transaminase mutant realizes the change of protein structure and functions, reduces the enzyme amount, increases the enantiomeric excess (ee) value of a product, and reduces the difficulty of post-processing, so that the transaminase mutant may be suitable for industrial production.