Patent · US Active

Linked ligation

US12410466B1 · kind B1 · utility

0Cited by
1References
20Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMar 18, 2025
Grant dateSep 9, 2025
Priority date—
Expiry dateMar 18, 2045

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q2535/122
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The invention generally relates to capturing, amplifying, and sequencing nucleic acids. In certain embodiments, copies of the sense and antisense strands of a duplex template nucleic acid are captured using linked capture probes and multiple binding and extension steps to improve specificity over traditional single binding target capture techniques. Methods of seeding sequencing clusters with sense and antisense strands of a target nucleic acid are also disclosed including identifying the strands using sense-specific barcodes and confirming base calls using two sense-specific sequencing reads. Linked adapters may be used to increase adapter ligation selectively or efficiency and yield.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.