Primer set of mycobacterium tuberculosis based on specific HRCA isothermal amplification and an application thereof
US12410486B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 18, 2025 |
| Grant date | Sep 9, 2025 |
| Priority date | — |
| Expiry date | Mar 18, 2045 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A primer set of Mycobacterium tuberculosis based on specific hyperbranched rolling circle amplification (HRCA) isothermal amplification includes: Lock and Key series primers for a recognition stage, and Loop and Link primers for an amplification stage. Using the primer set for an HRCA reaction enables rapid and sensitive detection with reliable and stable results. A method for detecting the Mycobacterium tuberculosis includes: (1) constructing a “lock” structure with recognition function; (2) performing magnetic selection and recognition; (3) conducting HRCA amplification; and (4) identifying a fluorescent signal to determine a positive or negative result determination. The method adopts the “lock” structure to design multiple conserved fragments of the Mycobacterium tuberculosis insertion sequence 6110 (IS6110) as target genes to jointly trigger the HRCA reaction, thereby enhancing the accuracy of the method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.