Method for membrane gas transfer in high density bioreactor culture
US12415980B2 · kind B2 · utility
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5References
15Claims
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Key dates
| Filing date | Oct 8, 2019 |
| Grant date | Sep 16, 2025 |
| Priority date | — |
| Expiry date | Nov 11, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M47/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides improved bioprocessing systems and methods for cell culture using the improved bioreactors, e.g., batch-fed or perfusion bioreactor cell culture systems for production of monoclonal or bi-specific antibodies, which are modified to include one or more membrane gas transfer modules in place of a sparger- or microsparger-based aeration systems to better regulate the levels of critical gases in a bioreactor cell culture, e.g., the dissolved levels of O2 and CO2, even at high cell densities, without subjecting the cells to bubble-burst associated cell death.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.